{"id":10712,"date":"2022-03-29T16:10:10","date_gmt":"2022-03-29T14:10:10","guid":{"rendered":"https:\/\/www.istitutopasteuritalia.it\/2022\/16\/contestabile-roberto\/"},"modified":"2026-08-26T11:46:05","modified_gmt":"2026-08-26T09:46:05","slug":"contestabile-roberto","status":"publish","type":"post","link":"https:\/\/www.istitutopasteuritalia.it\/en\/2022\/16\/contestabile-roberto\/","title":{"rendered":"Contestabile Roberto"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"10712\" class=\"elementor elementor-10712 elementor-5495\" data-elementor-post-type=\"post\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-3b2f5c72 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"3b2f5c72\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-25333428\" data-id=\"25333428\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-b44d30a elementor-widget elementor-widget-spacer\" data-id=\"b44d30a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-90de0e1 elementor-widget elementor-widget-heading\" data-id=\"90de0e1\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h1 class=\"elementor-heading-title elementor-size-default\">Roberto Contestabile<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-38160b2 elementor-position-left elementor-vertical-align-middle elementor-widget elementor-widget-image-box\" data-id=\"38160b2\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image-box.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-image-box-wrapper\"><figure class=\"elementor-image-box-img\"><img fetchpriority=\"high\" decoding=\"async\" width=\"540\" height=\"540\" src=\"https:\/\/www.istitutopasteuritalia.it\/wp-content\/uploads\/2022\/03\/Contestabile.png\" class=\"attachment-full size-full wp-image-10710\" alt=\"\" srcset=\"https:\/\/www.istitutopasteuritalia.it\/wp-content\/uploads\/2022\/03\/Contestabile.png 540w, https:\/\/www.istitutopasteuritalia.it\/wp-content\/uploads\/2022\/03\/Contestabile-150x150.png 150w, https:\/\/www.istitutopasteuritalia.it\/wp-content\/uploads\/2022\/03\/Contestabile-300x300.png 300w, https:\/\/www.istitutopasteuritalia.it\/wp-content\/uploads\/2022\/03\/Contestabile-359x359.png 359w, https:\/\/www.istitutopasteuritalia.it\/wp-content\/uploads\/2022\/03\/Contestabile-60x60.png 60w\" sizes=\"(max-width: 540px) 100vw, 540px\" \/><\/figure><div class=\"elementor-image-box-content\"><h3 class=\"elementor-image-box-title\">Full professor of Molecular Biology  <\/h3><p class=\"elementor-image-box-description\">roberto.contestabile@uniroma1.it<\/p><\/div><\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-dcc9085 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"dcc9085\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-e7ebeae\" data-id=\"e7ebeae\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-261e298 elementor-widget elementor-widget-heading\" data-id=\"261e298\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\">Academic Appointments\n<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-c0c85ff elementor-widget elementor-widget-text-editor\" data-id=\"c0c85ff\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p class=\"p1\">Coordinator of the Master\u2019s degree in Biochemistry, Sapienza University of Rome, Italy.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-6e21fab elementor-widget elementor-widget-heading\" data-id=\"6e21fab\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\">Scientific interests<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-6db4baf elementor-widget elementor-widget-text-editor\" data-id=\"6db4baf\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p class=\"p1\">Regulation of vitamin B6 metabolism in bacteria and humans. Neurological disorders related to vitamin B6 metabolism. Enzyme inhibition as a mean of chemotherapy intervention.  <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-f48989c elementor-widget elementor-widget-heading\" data-id=\"f48989c\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\">Contribution to Science<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1e016b1 elementor-widget elementor-widget-text-editor\" data-id=\"1e016b1\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p class=\"p1\">My research activity has mainly concerned pyridoxal phosphate-dependent enzymes and vitamin B6 metabolism in bacteria and humans. Concerning PLP-dependent enzymes, I have dealt with their catalytic mechanism, catalytic promiscuity and evolution of catalytic activities, the design and experimentation of specific inhibitors. Concerning vitamin B6 metabolism, I have contributed to the understanding of transcriptional regulation of vitamin B6 biosynthesis in bacteria and of the molecular basis of neurological disorders related to vitamin B6 metabolism. At present, my research activity is focused on the study of the pyridoxal 5\u2019-phosphate (PLP) binding protein (PLPBP), an important regulatory component of PLP biosynthesis and salvage pathways in bacteria and humans whose function is unknown. I am also involved in the design and testing of inhibitors of vitamin B6 metabolism as means of chemotherapy intervention.    <\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-92f78cb elementor-widget elementor-widget-heading\" data-id=\"92f78cb\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<p class=\"elementor-heading-title elementor-size-default\">5 Selected publications<\/p>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-3083732 elementor-widget elementor-widget-text-editor\" data-id=\"3083732\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<ul class=\"ul1\"><li class=\"li1\">Graziani C, Barile A, Parroni A, di Salvo ML, De Cecio I, Colombo T, Babor J, de Cr\u00e9cy-Lagard V, Contestabile R, Tramonti A. The ubiquitous pyridoxal 5&#8242;-phosphate-binding protein is also an RNA-binding protein. Protein Sci. 2024 Dec;33(12):e5242. doi: 10.1002\/pro.5242. PMID: 39604152<\/li><li class=\"li1\">Graziani C, Barile A, Antonelli L, Fiorillo A, Ilari A, Vetica F, di Salvo ML, Paiardini A, Tramonti A, Contestabile R. The Z isomer of pyridoxilidenerhodanine 5&#8242;-phosphate is an efficient inhibitor of human pyridoxine 5&#8242;-phosphate oxidase, a crucial enzyme in vitamin B6 salvage pathway and a potential chemotherapeutic target. FEBS J. 2024 Nov;291(22):4984-5001. doi: 10.1111\/febs.17274. Epub 2024 Sep 17. PMID: 39288205<\/li><li class=\"li1\">Malatesta M, Fornasier E, Di Salvo ML, Tramonti A, Zangelmi E, Peracchi A, Secchi A, Polverini E, Giachin G, Battistutta R, Contestabile R, Percudani R. One substrate many enzymes virtual screening uncovers missing genes of carnitine biosynthesis in human and mouse. Nat Commun. 2024 Apr 13;15(1):3199. doi: 10.1038\/s41467-024-47466-3. PMID: 38615009<\/li><li class=\"li1\">Tramonti A, Donkor AK, Parroni A, Musayev FN, Barile A, Ghatge MS, Graziani C, Alkhairi M, AlAwadh M, di Salvo ML, Safo MK, Contestabile R. Functional and structural properties of pyridoxal reductase (PdxI) from Escherichia coli: a pivotal enzyme in the vitamin B6 salvage pathway. FEBS J. 2023 Dec;290(23):5628-5651. doi: 10.1111\/febs.16962. Epub 2023 Oct 2. PMID: 37734924<\/li><li class=\"li1\">Tramonti A, Ghatge MS, Babor JT, Musayev FN, di Salvo ML, Barile A, Colotti G, Giorgi A, Paredes SD, Donkor AK, Al Mughram MH, de Cr\u00e9cy-Lagard V, Safo MK, Contestabile R. Characterization of the Escherichia coli pyridoxal 5&#8242;-phosphate homeostasis protein (YggS): Role of lysine residues in PLP binding and protein stability. Protein Sci. 2022 Nov;31(11):e4471. doi: 10.1002\/pro.4471. PMID: 36218140<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-ed18a22 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"ed18a22\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-51be655\" data-id=\"51be655\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-f77c831 elementor-widget elementor-widget-heading\" data-id=\"f77c831\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h4 class=\"elementor-heading-title elementor-size-default\">On-going Grants <\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7e16126 elementor-widget elementor-widget-html\" data-id=\"7e16126\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"html.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<table class=\"ricercatori-table\">\n    <tr class=\"ricercatori-table-head\">\n        <td>Project Title <\/td>\n        <td>Funding source <\/td>\n        <td>Amount (Euros) <\/td>\n        <td>Period <\/td>\n        <td>Role of the PI <\/td> \n    <\/tr>\n    <tr class=\"ricercatori-table-row\">\n        <td>The enigmatic Escherichia coli PLP-binding protein (YggS) is an RNA-binding protein - Investigating the structural and functional implications of this novel observation. <\/td>\n<td>Istituto Pasteur-Fondazione Cenci Bolognetti <\/td>\n<td>40.000 <\/td>\n<td>1\/3\/2025\u201328\/02\/2027 <\/td>\n<td>PI (30 % of time) <\/td> \n    <\/tr>\n    \n<\/table>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ca88149 elementor-widget elementor-widget-text-editor\" data-id=\"ca88149\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Complete list of published work in MyBibliography:<\/strong><br\/><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=contestabile+r\"><span style=\"text-decoration: underline;\">https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=contestabile+r<\/span><\/a><br\/><\/p><p><strong>KEYWORDS<\/strong><br\/>Vitamin B6 metabolism; RNA binding proteins; pyridoxal 5&#8242;-phosphate; PLP-binding protein<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Roberto Contestabile Full professor of Molecular Biology roberto.contestabile@uniroma1.it Academic Appointments Coordinator of the Master\u2019s degree in Biochemistry, Sapienza University of Rome, Italy. Scientific interests Regulation of vitamin B6 metabolism in bacteria and humans. Neurological disorders related to vitamin B6 metabolism. Enzyme inhibition as a mean of chemotherapy intervention. Contribution to Science My research activity has &hellip;<\/p>\n","protected":false},"author":2,"featured_media":10711,"comment_status":"open","ping_status":"open","sticky":false,"template":"elementor_header_footer","format":"standard","meta":{"give_campaign_id":0,"footnotes":""},"categories":[192],"tags":[],"class_list":["post-10712","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-sapienza-researchers-funded-by-istituto-pasteur"],"_links":{"self":[{"href":"https:\/\/www.istitutopasteuritalia.it\/en\/wp-json\/wp\/v2\/posts\/10712","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.istitutopasteuritalia.it\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.istitutopasteuritalia.it\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.istitutopasteuritalia.it\/en\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.istitutopasteuritalia.it\/en\/wp-json\/wp\/v2\/comments?post=10712"}],"version-history":[{"count":6,"href":"https:\/\/www.istitutopasteuritalia.it\/en\/wp-json\/wp\/v2\/posts\/10712\/revisions"}],"predecessor-version":[{"id":11512,"href":"https:\/\/www.istitutopasteuritalia.it\/en\/wp-json\/wp\/v2\/posts\/10712\/revisions\/11512"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.istitutopasteuritalia.it\/en\/wp-json\/wp\/v2\/media\/10711"}],"wp:attachment":[{"href":"https:\/\/www.istitutopasteuritalia.it\/en\/wp-json\/wp\/v2\/media?parent=10712"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.istitutopasteuritalia.it\/en\/wp-json\/wp\/v2\/categories?post=10712"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.istitutopasteuritalia.it\/en\/wp-json\/wp\/v2\/tags?post=10712"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}